Analysis of gene expression induced by diethylstilbestrol (DES) in human primitive Müllerian duct cells using microarray

The Müllerian ducts are strongly influenced by natural estrogen, estradiol (E2) and diethylstilbestrol (DES) in their development. We screened E2 and DES responsive genes using a microarray analysis in human primitive Müllerian duct cell line, EMTOKA cells expressed estrogen receptor (ER) β. c-myc oncogene and other target genes expression was detected in cells treated by high-dose DES, but ER antagonist ICI 182,780 could not prevent c-myc induction above. Results of our present study suggested the presence of ER independent pathway in oncogenes induction process by high-dose DES treatment in a human primitive Müllerian duct cell line.