A mini me?: Exploring early childhood diet with stable isotope ratio analysis using primary teeth dentin

AbstractObjectives truct childhood diet using teeth collected in Edmonton in a collaborative effort between the Departments of Dentistry and Anthropology at the University of Alberta. s ous teeth needing extraction were collected from 33 children for stable isotope ratio analysis of diet. Tooth dentin was microsampled in three locations using a newly developed technique to reconstruct the changing pattern of participants’ diet through early childhood including breastfeeding practices. s crosampling method can reconstruct diet with tiny samples (0.3 mg). The results reconstruct fetal isotope ratios, which showed significant variation. δ15N values indicate some children were being breastfed (7/17), while others were likely bottle fed (10/17). Surprisingly, the early childhood results do not show the range of diets expected in adults based on known eating habits. Toddler diets form a tight cluster implying diets of similar isotopic composition in almost all of the households despite potential cultural and class distinctions (δ15N values 11–11.5‰, δ13C values around −18‰). The δ13C values show a strong C3 dependence for most children, a two outliers show C4 (−12‰) dependence indicating a possible corn based diet. sions ampling can potentially track each childʹs diet through early childhood. For this group of children, both breastfeeding and bottle feeding was practiced. However, the percent of breastfed infants was less than reported Canadian rates. Surprisingly, the choice to breastfeed or to bottle feed was not linked to the choice of toddler diet. All toddler diets were narrower in scope than adult diets.