Author/Authors :
Michaelis، نويسنده , , Martin and Cinatl، نويسنده , , Jaroslav and Anand، نويسنده , , Puja and Rothweiler، نويسنده , , Florian and Kotchetkov، نويسنده , , Rouslan and Deimling، نويسنده , , Andreas von and Doerr، نويسنده , , Hans W. and Shogen، نويسنده , , Kuslima and Cinatl Jr.، نويسنده , , Jindrich، نويسنده ,
Abstract :
The efficacy of Onconase on the growth of a panel of chemosensitive and chemoresistant neuroblastoma cell lines was investigated. Onconase decreased cell viability of chemosensitive (IMR-32, UKF-NB-3) and chemoresistant neuroblastoma cell lines characterised by high expression of P-glycoprotein (P-gp) (UKF-NB-3rDOX20) or by high P-gp expression in combination with mutated p53 (UKF-NB-3rVCR10, Be(2)-C), in a similar manner. Moreover, Onconase caused cell cycle block in G1 phase and induced caspase-independent cell death. Transmission electron microscope investigations suggested that Onconase-induced autophagy contributes to Onconase-induced cell death. Antitumour activity of Onconase against naïve and drug-resistant neuroblastoma xenografts was confirmed in animals.
Keywords :
onconase , Neuroblastoma , Multi-drug-resistance , P-GLYCOPROTEIN , p53
