Title of article :
Abnormal expression, highly efficient detection and novel truncations of midkine in human tumors, cancers and cell lines
Author/Authors :
Tao، نويسنده , , Ping and Xu، نويسنده , , Donghui and Lin، نويسنده , , Shuibin and Ouyang، نويسنده , , Gao-Liang and Chang، نويسنده , , Youde and Chen، نويسنده , , Qin and Yuan، نويسنده , , Yuanyang and Zhuo، نويسنده , , Xinming and Luo، نويسنده , , Qicong and Li، نويسنده , , Jie and Li، نويسنده , , Baoan and Ruan، نويسنده , , Lingjuan and Li، نويسنده , , Qifu and Li، نويسنده , , Zhixing، نويسنده ,
Abstract :
We detected aberrant Midkine (MK) expressions in human insulinoma and pancreatic cancer tissues by immunohistochemistry, revealing its potential role in tumorigenesis/carcinogenesis. With a nested-touchdown PCR program we were able to detect the tMK in all human tumor/cancer tissues and cancer/tumor cell lines. Detection of MK in the peripheral cells and precancerous lesions implies its potential for early cancer/tumor diagnosis. Furthermore, we have discovered two novel truncations of the MK, tMKB and tMKC, respectively, in the disease specimens. Our data not only provide an efficient methodology potentially for clinical application but also shed light on the molecular mechanism underlying the role for MK in tumorigenesis/carcinogenesis.
Keywords :
Truncated midkine , Tumorigenesis/carcinogenesis , Tumor makers , Alternative splicing , Nested-touchdown PCR
