Author/Authors :
SEPEHRI، H نويسنده Animal Physiology Department, School of Biology, College of Science, University of Tehran, I. R. of Iran , , DELPHI، L نويسنده Animal Physiology Department, School of Biology, College of Science, University of Tehran, I. R. of Iran , , RASSOULI، Y نويسنده Animal Physiology Department, School of Biology, College of Science, University of Tehran, I. R. of Iran ,
Abstract :
It is well known that several plant extracts have a lactogenic effect. This response may be induced
through the stimulation of prolactin release from lactotrope cells. A partial purification of the active
compound indicated that it is rich in pectin and B-glucan. These materials stimulate secretion of prolactin
from hypophyseal fragments. In the present study, the effect of B-Glucan on the secretion of prolactin from
GH3/B6 pituitary tumor cells has been investigated. These cells are good tools for studying the direct effect of
B-glucan on prolactin secretion. The different concentrations of B-glucan (50, 100 and 200 µg/ml) increased
secretion of prolactin within 24 hours of incubation in GH3/B6 media. Moreover, the secretion of prolactin
was significantly elicited at 100 and 200 µg/ml of B-glucan incubation after 48 hours. These results suggest
that B-glucan can affect the synthesis and/or release of prolactin, a hormone that plays many important
physiological roles from GH3/B6 pituitary tumor cells.
