Author/Authors :
Li، نويسنده , , Dan-juan and Deng، نويسنده , , Gui and Xiao، نويسنده , , Zhi-qiang and Yao، نويسنده , , Hui-xin and Li، نويسنده , , Cui and Peng، نويسنده , , Fang and Li، نويسنده , , Mao-yu and Zhang، نويسنده , , Pengfei and Chen، نويسنده , , Yongheng and Chen، نويسنده , , Zhuchu، نويسنده ,
Abstract :
In this study, we aim to screen metastasis-related proteins in human lung squamous carcinoma (LSC) using laser capture microdissection and a proteomic approach. Twenty two differential proteins were identified from pooled microdissected primary LSC and matched lymph node (LN) metastatic tissues. Expression of the differential protein 14-3-3 sigma was determined by Western blotting and immunohistochemistry. In cell invasion assay, down-regulated 14-3-3 sigma by siRNA increased in vitro invasive ability of HTB-182 and A549 cells, up-regulation of 14-3-3 sigma by pcDNA3.0/14-3-3 sigma decreased in vitro invasive ability of HTB-182 and A549 cells. The data suggest that 14-3-3 sigma is a potential LN metastasis-related protein in LSC, and its dysregulation might play an important role in the LN metastatic process of LSC.
Keywords :
Lymph node metastasis-related protein , 14-3-3 Sigma , Lung squamous carcinoma , PROTEOMICS
