Author/Authors :
Mohan، K. Murali نويسنده , , Peddanna، K. نويسنده DST-PURSE, Sri Venkateswara University, Tirupati, Andhra Pradesh. INDIA , , Ramya، A. نويسنده Department of Microbiology, Sri Krishnadevaraya University, Anantapur. Andhra Pradesh. INDIA. , , Lakka، Vinaysagar نويسنده Department of Biotechnology, Akshaya Biological Corporation. Himayat nagar, Hyderabad. INDIA. , , Rayalu Daddam، Jayasimha نويسنده Department of Biotechnology, Akshaya Biological Corporation, Himayath Nagar, Hyderabad ,
Abstract :
Diabetes mellitus type 2 is a metabolic disorder that is characterized by high blood glucose in the context of insulin resistance and relative insulin deficiency. A number of genes are involved in regulating lipid metabolism and insulin sensitivity, and thereby affecting the susceptibility to type 2 diabetes mellitus. Among them is the gene which is responsible for Sterol Response Element Binding Protein-1 (SREBP). SREBPs are transcription factors that bind to the sterol regulatory element DNA sequence TCACNCCAC. Mammalian SREBPs are encoded by the genes SREBF1 and SREBF2. In this work A 3D model of SREBP was generated using 2OVP (Crystal structure of Fbw7-Skp1-Cyclin E complex) as template with the help of Modeller7v7. With the aid of the molecular mechanics and molecular dynamics methods, the final model is obtained and is further assessed by Procheck and Verify 3D graph programs, which showed that the final refined model is reliable. After energy minimization study this structure was used for binding with DNA. We identified 35 genes coding for SREBP-1 and their locus regions. Multiple alignment and phylogenetic analysis showed that the total SREBP family proteins formed 6 branches. From the result we identified inhibitory region of the SREBP-1 protein while binding to DNA.
