Author/Authors :
Marchetti، نويسنده , , C. and Migliorati، نويسنده , , G. and Moraca، نويسنده , , R. and Riccardi، نويسنده , , Sheila C. and Nicoletti، نويسنده , , I. and Fabiani، نويسنده , , R. and Mastrandrea، نويسنده , , V. and Morozzi، نويسنده , , G.، نويسنده ,
Abstract :
Short chain fatty acids (propionate and butyrate) and deoxycholic acid (DCA) are able to induce apoptosis in HT-29 colonic tumor cell line, but DCA induces a much higher level of apoptosis than butyrate and propionate. Mixtures of DCA with butyrate or propionate enhance the effect of the single components. Apoptosis is not affected by the PKC, PTK or de novo mRNA and protein synthesis inhibitors, so that the involvement of these enzymes and processes is ruled out. In contrast, DCA-induced apoptosis is directly related to [Ca2+]i concentration as demonstrated by the apoptosis inhibition caused by [Ca2+]i chelator BAPTA/AM.
