Title of article :
Induction of telomerase activity during regeneration after partial hepatectomy in the rat
Author/Authors :
Tsujiuchi، نويسنده , , Toshifumi and Tsutsumi، نويسنده , , Masahiro and Kido، نويسنده , , Akira and Takahama، نويسنده , , Makoto and Sakitani، نويسنده , , Hiroyuki and Iki، نويسنده , , Katsumichi and Sasaki، نويسنده , , Yasutaka and Denda، نويسنده , , Ayumi and Konishi، نويسنده , , Yoichi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1998
Pages :
6
From page :
115
To page :
120
Abstract :
The regulation of telomerase activity during regeneration induced by two-thirds partial hepatectomy (PH) was investigated in 6-week-old male F344 rats. Groups of animals were serially sacrificed 0, 6, 16, 24, 36 and 72 h after the operation and telomerase activity was determined by the telomeric repeat amplification protocol (TRAP) assay followed by densitometric quantification. DNA synthesis was immunohistochemically quantified in terms of bromodeoxyuridine (BrdU) incorporation. The expression levels of telomerase RNA were examined by Northern blot analysis. Telomerase activity was increased significantly from 6 to 36 h but had decreased to close to the normal levels after 72 h. DNA synthesis reached a maximum 24 h after PH. However, the expression levels of telomerase RNA did not change during regeneration. The results suggest that telomerase is actively regulated by unknown mechanisms throughout the cell cycle in regenerating rat hepatocytes.
Keywords :
Telomerase , TRAP ASSAY , Partial hepatectomy , Liver regeneration , Rat
Journal title :
Cancer Letters
Serial Year :
1998
Journal title :
Cancer Letters
Record number :
1815844
Link To Document :
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