Author/Authors :
Abolhasani، Marziyeh Marziyeh نويسنده Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Abolhasani, Marziyeh Marziyeh , Hashemzadeh Chaleshtori، Morteza نويسنده , , Doosti، Abbas نويسنده Department of Biology, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran Doosti, Abbas , Amini Sarteshnizi، Nematolah نويسنده Department of Biology, University of Mohaghegh Ardabili, Ardabil, Iran Amini Sarteshnizi, Nematolah , Gholami-Arjenaki، Mostafa نويسنده Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Gholami-Arjenaki, Mostafa , Teimori، Hossein نويسنده Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Teimori, Hossein
Abstract :
Introduction: The Ras/Akt/mTORC1 signal transduction pathways play a critical role in regulating translation and converge on initiation factor eukaryotic translation initiation factor 4E (eIF4E) which is overexpressed in various malignancies. In the current study we aimed to assess the effect of chrysin and caffeic acid phenethyl ester (CAPE) on eIF4E expression level in human stomach cancer AGS cell line.
Methods: AGS cells were treated with 15, 20, 30 and 40 ?M concentration of chrysin and CAPE separately, then eIF4E expression was evaluated in treated cells using real time-PCR method.
Results: A significant decrease in eIF4E expression in the cells following 40 ?M chrysin treatment was observed (p < 0.05). There was a significant decrease in CAPE-treated cells in a dose dependent manner. Indeed the cells treated with 30 and 40 ?M concentrations of CAPE, showed a significant decline in eIF4E expression (p < 0.05).
Conclusion: Our results suggest that CAPE and chrysin may be useful as a potential therapeutic agent for treatment of gastric cancers with an elevated eIF4E expression level.
