Author/Authors :
Iolascon، نويسنده , , Achille and Lo Cunsolo، نويسنده , , Crocifissa and Giordani، نويسنده , , Lucia and Cusano، نويسنده , , Roberto and Mazzocco، نويسنده , , Katia and Boumgartner، نويسنده , , Martina and Ghisellini، نويسنده , , Paola and Faienza، نويسنده , , Maria Felicia and Boni، نويسنده , , Luca and De Bernardi، نويسنده , , Bruno and Conte، نويسنده , , Massimo and Romeo، نويسنده , , Giovanni and Tonini، نويسنده , , Gian Paolo، نويسنده ,
Abstract :
We studied chromosome 1p loss of heterozygosity (1p-LOH) in 53 neuroblastomas (NBs) using 15 (CA)n repeat loci, which covered a region of 90 cM. We also assessed chromosome 1p36 deletion by fluorescence in situ hybridization (FISH) on interphase nuclei. 1p-LOH was found in 19 (36%, 95% confidence interval (CI) 23–50%) NBs. We detected interstitial and large deletion in both localized and disseminated tumours and in one tumour of a patient at stage 4S. Allelic loss was frequently observed in 1p36 and 1p32 regions. In patients older than 1 year of age (53 versus 13%, P<0.002) we detected significant chromosome 1p deletion and it was associated with MYCN amplification (P=0.001). Overall survival (OS) analysis showed that 1p-LOH is predictive of a poor outcome (odds ratio 16.5, 95% CI 5.4–50.9%); therefore, 1p-LOH should be regarded as an additional tumour progression marker in neuroblastoma.
Keywords :
Microsatellite , Neuroblastoma , Chromosome 1 , Loss of Heterozygosity , MYCN amplification
