Author/Authors :
Ando، نويسنده , , Soichiro and Nojima، نويسنده , , Kumie and Majima، نويسنده , , Hideyuki and Ishihara، نويسنده , , Hiroshi and Suzuki، نويسنده , , Masao and Furusawa، نويسنده , , Yoshiya and Yamaguchi، نويسنده , , Hiroshi and Koike، نويسنده , , Sachiko and Ando، نويسنده , , Koichi and Yamauchi، نويسنده , , Masatake and Kuriyama، نويسنده , , Takayuki، نويسنده ,
Abstract :
Vascular endothelial growth factor (VEGF) is a multipotent cytokine which plays an important role in various angiogenic conditions as well as in some tumor behaviors. Here we examined the induction of VEGF mRNA by X-ray irradiation in a lung squamous cell carcinoma cell line (RERF-LC-AI). Irradiating the cells with 15 Gy X-rays significantly increased the mRNA expression up to 2.5-fold of control at a post-irradiation time of 16–24 h. The induction of VEGF mRNA by X-ray irradiation was completely blocked by treating cells with either genistein (Src tyrosine kinase inhibitor) or H7 (protein kinase C inhibitor). This suggests that the mechanism of induction might be concerned with the pathway which triggers Src tyrosine kinase of the cell surface and the protein kinase C pathway.
Keywords :
Vascular endothelial growth factor , X-ray irradiation , Src tyrosine kinase , Protein kinase C
