Author/Authors :
Kobayashi، نويسنده , , Takahiko and Honke، نويسنده , , Koichi and Tsunematsu، نويسنده , , Izumi and Kagaya، نويسنده , , Hidetoshi and Nishikawa، نويسنده , , Shuji and Hokari، نويسنده , , Kaku and Kato، نويسنده , , Mototsugu and Takeda، نويسنده , , Hiroshi and Sugiyama، نويسنده , , Toshiro and Higuchi، نويسنده , , Akifumi and Asaka، نويسنده , , Masahiro، نويسنده ,
Abstract :
Sulfatide is a major acidic glycolipid in human gastric mucosa, and its sulfation is catalyzed by cerebroside sulfotransferase (CST). To investigate the expression of the CST gene in human gastric cancer, a reverse transcription PCR method was developed with the use of endoscopic bioptic specimens. By this method, we examined the CST mRNA expression in 11 cases of gastric cancer, and in all the cases we detected various levels of the expression both in cancer tissues and in uninvolved adjacent tissues. The present assay method was suggested to be useful in the detection of CST mRNA from a limited amount of bioptic samples.
Keywords :
Sulfatide , Gastric cancer , sulfotransferase , Glycolipid
