Title of article
MOLECULAR IDENTIFICATION OF PERGULARIA DAEMIA (FORSSK.) CHIOV. USING TRNL INTRON-TRNL- F CHLOROPLAST GENOME
Author/Authors
Raghavamma، S.T.V. نويسنده Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, AP, India. , , Rao، N.Rama نويسنده Chalapathi Institute of pharmaceutical sciences, Guntur, Andhra Pradesh, India. , , Rao، K.R.S.Sambasiva نويسنده Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, AP, India. , , DevelaRao، G. نويسنده KVSR Sidhartha College of Pharmaceutical Sciences, Vijayawada ,
Issue Information
فصلنامه با شماره پیاپی سال 2013
Pages
8
From page
37
To page
44
Abstract
Pergularia daemia (Forssk) chivo is a shrub of Asclepiadaceae family found in the plains throughout India and in tropical countries worldwide. Asclepiadaceae members are known for their rich source of glucosides. The present work is taken up to identify the plant species at molecular level. The chloroplast trnl- F region was amplified from extracted total genomic DNA using the polymerase chain reaction (PCR) and sequenced. Sequence of the dominant DGGE band revealed that Pergularia daemia in the tested leaves was Pergularia daemia (100% similarity to ITS sequence of Pergularia daemia). This sequence was deposited in NCBI with the accession no. JN205300.
Journal title
International Journal of Analytical, Pharmaceutical and Biomedical Sciences
Serial Year
2013
Journal title
International Journal of Analytical, Pharmaceutical and Biomedical Sciences
Record number
1817364
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