Author/Authors :
Chandra، Raju نويسنده Department of Pharmaceutical Chemistry, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun-248001, Uttarakhand, India , , Kumar، Sujeet نويسنده Department of Pharmaceutical Chemistry, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun-248001, Uttarakhand, India , , SINGH، SANJAY KUMAR نويسنده , , Sharma، Keshav Dutt نويسنده Department of Pharmaceutical Chemistry, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun-248001, Uttarakhand, India , , Alam ، Md. Naushad نويسنده Department of Pharmaceutical Chemistry, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun-248001, Uttarakhand, India , , Verma، Daleep نويسنده Department of Pharmaceutical Chemistry, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun-248001, Uttarakhand, India ,
Abstract :
Assay is an important method for the accurate quantity determination of drug analyte in the formulated products. For which a rapid simple sensitive isocratic reproducible reversed phase-high performance liquid chromatographic technique has been developed for the quantitative assay evaluation of ascorbic acid. The chromatographic separation of ascorbic acid samples were performed on a C18-column by isocratic elusion at the column 250C. The mobile phase was used methanol and water in the ratio of 50:50 (v/v). The flow rate was 1.0 ml/min. The maximum peak area was resolved at 260 nm. The limit of detection and limit of quantification were 0.031 and 0.093 µg/mL by RP-HPLC and 0.3 and 0.9 µg/mL by UV-Spectrophotometry, respectively. Good results were obtained with respect to linearity R2=0.997 by RP-HPLC and R2=0.983 by UV-Spectrophotometry. The mean recoveries in inter-day and intra-day for RP-HPLC were calculated 99.98 % and 100.2 % and for UV-Spectrophotometry 99.08 % and 99.37 %. The method was validated for linearity, accuracy, repeatability, limit of detection (LOD) and limit of quantification (LOQ). The method is simple, accurate and precise and was successfully applied to the determination of ascorbic acid from formulated products.
