Author/Authors :
Herrmann، نويسنده , , Marille E. and Lalley، نويسنده , , Peter A. and Rydstedt، نويسنده , , Linea L. and Meese، نويسنده , , Eckart and Lang، نويسنده , , Charles H. and Abumrad، نويسنده , , Naji N. and Moll، نويسنده , , Ute M. and Talpos، نويسنده , , Gary B.، نويسنده ,
Abstract :
The cell line PTC-1113A was established from a metastasizing recurrent papillary thyroid cancer. The cell line was growing as monolayer and showed a complex karyotype with chromosome numbers ranging from 30 to 140/metaphase. A proportion of metaphases contained double minutes and/or pulverized chromosomes. Extrachromosomal DNA seemed to originate from a B-group chromosome. A chromosome 4 painting probe hybridized to extrachromosomal material, representing double minutes (dmin) and possibly minutes. In addition, fluorescence in situ hybridization (FISH) with the chromosome 4 library detected a translocation chromosome and a pulverized chromosome originating from chromosome 4. PTC-1113A is, to our knowledge, the single papillary thyroid cancer cell line demonstrating evidence of gene amplification.
