Author/Authors :
Jang، نويسنده , , Su Jin and Kang، نويسنده , , Joo-Hyun and Kim، نويسنده , , Kwang Il and Lee، نويسنده , , Tae Sup and Lee، نويسنده , , Yong Jin and Lee، نويسنده , , Kyo Chul and Woo، نويسنده , , Kwang Sun and Chung، نويسنده , , Wee Sup and Kwon، نويسنده , , Hee Chung and Ryu، نويسنده , , Chun Jeih and Choi، نويسنده , , Tae Hyun and Choi، نويسنده , , Chang Woon and Lim، نويسنده , , Sang Moo and Cheon، نويسنده , , Gi Jeong، نويسنده ,
Abstract :
Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [125I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [18F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.
Keywords :
luciferase , molecular imaging , Suicidal gene therapy , Glioma , HSV1-TK
