Title of article
Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1
Author/Authors
Fernلndez-Martيnez، نويسنده , , Ana B. and Bajo، نويسنده , , Ana M. and Isabel Arenas، نويسنده , , M. and Sلnchez-Chapado، نويسنده , , Manuel Hermida-Prieto، نويسنده , , Juan C. and Carmena، نويسنده , , Marيa J.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2010
Pages
11
From page
11
To page
21
Abstract
The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts. VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases MMP-2 and MMP-9, decreased E-cadherin-mediated cell–cell adhesion, and increased cell motility. VIP increased cyclin D1 expression and cell proliferation that was blocked after VPAC1-receptor siRNA transfection. Similar effects were seen in RWPE-1 tumors developed by subcutaneous injection of VIP-treated cells in athymic nude mice. VIP acts as a cytokine in RWPE-1 cell transformation conceivably through epithelial–mesenchymal transition (EMT), reinforcing VIP role in prostate tumorigenesis.
Keywords
VIP , RWPE-1 cells , EMT , tumorigenesis , prostate cancer
Journal title
Cancer Letters
Serial Year
2010
Journal title
Cancer Letters
Record number
1819330
Link To Document