Author/Authors :
Vicinus، نويسنده , , Benjamin and Rubie، نويسنده , , Claudia and Faust، نويسنده , , Sabrina K. and Frick، نويسنده , , Vilma Oliveira and Ghadjar، نويسنده , , Pirus and Wagner، نويسنده , , Mathias and Graeber، نويسنده , , Stefan and Schilling، نويسنده , , Martin K.، نويسنده ,
Abstract :
As deregulation of miRNAs and chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis, we analyzed the functional interactions of candidate miRNAs with CCL20 mRNA. After target prediction software programs indicated a role for miR-21 in CCL20 regulation, we applied the luciferase reporter assay system to demonstrate that miR-21 functionally interacts with the 3′UTR of CCL20 mRNA and down-regulates CCL20 in miR-21 mimic transfected CRC cell lines (Caco-2, SW480 and SW620). Thus, regulation of CCL20 expression by miR-21 might be a regulatory mechanism involved in progression of CRC.
Keywords :
MicroRNA , miR-21 , CCL20 , CRC , chemokines
