Author/Authors :
Wang، نويسنده , , Shuhui and Chen، نويسنده , , Changchun and Meng، نويسنده , , Yanchun and Hu، نويسنده , , Shi and Zheng، نويسنده , , Lei and Song، نويسنده , , Jinjing and Zhang، نويسنده , , Dapeng and Li، نويسنده , , Bohua and Guo، نويسنده , , Yajun، نويسنده ,
Abstract :
Despite the effectiveness of the anti-ErbB2 humanized antibody trastuzumab, less than 35% of patients with ErbB2-overexpressing breast cancer respond to the treatment. Here we engineered an anti-EGFR/ErbB2 bispecific antibody (TC-BsAb) using trastuzumab and cetuximab, an anti-EGFR chimeric antibody. TC-BsAb treatment led to internalization of both EGFR and ErbB2, whereas trastuzumab and cetuximab, either alone or in combination, failed to induce ErbB2 internalization. Both in vitro and in vivo experiments indicated that TC-BsAb was significantly more potent in inhibiting the growth of breast cancer cell lines than trastuzumab, cetuximab, and trastuzumab plus cetuximab, suggesting its potential use for treating breast cancer.
Keywords :
bispecific , erbB2 , EGFR , monoclonal antibody , breast cancer
