Title of article
A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells
Author/Authors
Takahashi، نويسنده , , Masayuki and Elbarbary، نويسنده , , Reyad A. and Nakashima، نويسنده , , Aiko and Abe، نويسنده , , Mayumi and Watanabe، نويسنده , , Norihiro and Narita، نويسنده , , Miwako and Takahashi، نويسنده , , Masuhiro and Tamura، نويسنده , , Masato and Yoshida، نويسنده , , Tetsuo and Nashimoto، نويسنده , , Masayuki، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
7
From page
362
To page
368
Abstract
tRNase ZL-utilizing efficacious gene silencing is a gene control technology, which is based on the property that tRNase ZL can cleave any target RNA under the direction of an appropriate small guide RNA (sgRNA). To find therapeutic sgRNAs to cure hematological malignancies, we investigated behavior of heptamer-type sgRNA. We demonstrated that a heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents and that it can induce apoptosis of the leukemia cells. Mouse xenograft experiments showed that a median survival of the mh1(Bcl-2)-treated mice was longer than that of the control mice.
Keywords
small guide RNA , leukemia , bcl-2 , tRNase ZL , TRUE gene silencing
Journal title
Cancer Letters
Serial Year
2013
Journal title
Cancer Letters
Record number
1822216
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