Author/Authors :
Takeo، نويسنده , , Saori and Arai، نويسنده , , Hiroshi and Kusano، نويسنده , , Noriyoshi and Harada، نويسنده , , Tomohiko and Furuya، نويسنده , , Tomoko and Kawauchi، نويسنده , , Shigeto and Oga، نويسنده , , Atsunori and Hirano، نويسنده , , Takashi and Yoshida، نويسنده , , Tomoharu and Okita، نويسنده , , Kiwamu and Sasaki، نويسنده , , Kohsuke، نويسنده ,
Abstract :
To identify amplified oncogenes involved in hepatocellular carcinomas (HCC), we applied a genomic DNA microarray spotted with 57 oncogenes to 20 HCCs. Aberrations in DNA copy number also were analyzed by comparative genomic hybridization (CGH) using an aliquot of DNA samples. In 5 of 20 HCCs, only 6 oncogenes (CCND1, FGF3/FGF4, SAS/CDK4, TERC, MET, and MYC) were amplified, whereas in the remaining 15 tumors no oncogenes were amplified. A comparison of DNA microarray and conventional CGH analyses showed that, although 5 of 6 amplified oncogenes shown by microarray were located in chromosomal regions shown by CGH to have increased DNA copy numbers, not all genes located in such chromosomal regions were affected. One of the amplified oncogenes (SAS/CDK4) was found in a chromosomal region that was undetected by CGH. We, therefore, conclude that amplification of the oncogenes examined in this series is not directly implicated in hepatocellular carcinogenesis.
