Author/Authors :
Yan، نويسنده , , Gui-Jun and Yu، نويسنده , , Fei and Wang، نويسنده , , Bin and Zhou، نويسنده , , Huai-Jun and Ge، نويسنده , , Qiu-Yan and Su، نويسنده , , Jing and Hu، نويسنده , , Ya-Li and Sun، نويسنده , , Haixiang and Ding، نويسنده , , Li-Jun، نويسنده ,
Abstract :
MicroRNA miR-302 has been found to induce some tumor cell lines to “transdifferentiate” into miRNA-induced pluripotent stem cells (mirPS), thereby inhibiting tumor cell proliferation and reducing tumorigenicity. This study firstly found that miR-302 inhibited the proliferation and migration of endometrial cell line, Ishikawa and HEC-1-B, and arrested cell cycle at the G2/M phase. In addition, miR-302 inhibited tumorigenicity in immunodeficient mice transplanted with Ishikawa cells. Microarray and Western blotting results showed that miR-302 significantly inhibited CDK1 and Cyclin D1 gene expression in Ishikawa cells. MiR-302 directly targeted Cyclin D1, but indirectly regulated CDK1 gene expression.
Keywords :
G2/M phase , HEC-1-B cells , MicroRNA miR-302 , Ishikawa cells
