Author/Authors :
Yasui، نويسنده , , Hironobu and Takeuchi، نويسنده , , Ryo and Nagane، نويسنده , , Masaki and Meike، نويسنده , , Shunsuke and Nakamura، نويسنده , , Yoshinari and Yamamori، نويسنده , , Tohru and Ikenaka، نويسنده , , Yoshinori and Kon، نويسنده , , Yasuhiro and Murotani، نويسنده , , Hiroki and Oishi، نويسنده , , Motoi and Nagasaki، نويسنده , , Yukio and Inanami، نويسنده , , Osamu، نويسنده ,
Abstract :
High atomic number molecules, such as gold and platinum, are known to enhance the biological effect of X-irradiation. This study was aimed to determine the radiosensitizing potential of PEGylated nanogel containing gold nanoparticles (GNG) and the cellular mechanism involved. GNG pretreatment increased the levels of reproductive cell death and apoptosis induced by X-irradiation. GNG accumulated in cytoplasm and increased the expression of endoplasmic reticulum (ER) stress-related protein. GNG suppressed the repair capacity of DNA after X-irradiation by down-regulating DNA repair-related proteins. Our results suggest that GNG radiosensitized cells by enhancing apoptosis and impairing DNA repair capacity via ER stress induction.
Keywords :
apoptosis , Endoplasmic reticulum stress , DNA repair , Gold nanoparticle , radiation
