Author/Authors :
Brehm، نويسنده , , Hannes and Niesen، نويسنده , , Judith and Mladenov، نويسنده , , Radoslav and Stein، نويسنده , , Christoph and Pardo، نويسنده , , Alessa and Fey، نويسنده , , Georg and Helfrich، نويسنده , , Wijnand and Fischer، نويسنده , , Rainer and Gattenlِhner، نويسنده , , Stefan and Barth، نويسنده , , Stefan، نويسنده ,
Abstract :
The treatment of rhabdomyosarcoma (RMS) remains challenging, with metastatic and alveolar RMS offering a particularly poor prognosis. Therefore, the identification and evaluation of novel antigens, which are suitable targets for immunotherapy, is one attractive possibility to improve the treatment of this disease. Here we show that chondroitin sulfate proteoglycan 4 (CSPG4) is expressed on RMS cell lines and RMS patient material. We evaluated the immunotoxin (IT) αMCSP-ETA′, which specifically recognizes CSPG4 on the RMS cell lines RD, FL-OH1, TE-671 and Rh30. It is internalized rapidly, induces apoptosis and thus kills RMS cells selectively. We also demonstrate the specific binding of this IT to RMS primary tumor material from three different patients.
Keywords :
CSPG4 , immunotoxin , immunotherapy , rhabdomyosarcoma
