Author/Authors :
Rudolph، نويسنده , , C. and Steinemann، نويسنده , , D. and von Neuhoff، نويسنده , , N. and Gadzicki، نويسنده , , D. and Ripperger، نويسنده , , T. and Drexler، نويسنده , , H.G. and Mrasek، نويسنده , , K. and Liehr، نويسنده , , T. and Claussen، نويسنده , , U. and Emura، نويسنده , , M. and Schrock، نويسنده , , E. and Schlegelberger، نويسنده , , B.، نويسنده ,
Abstract :
Combining fluorescence R-banding, fluorescence in situ hybridization and spectral karyotyping allowed us to precisely define chromosomal breakpoints, gains, losses and a newly detected amplification in the human mantle cell lymphoma (MCL) cell line GRANTA-519. GRANTA-519 is characterized by the t(11;14)(q13;q32) resulting in overexpression of cyclin D1, a key player in cell cycle control. Hitherto unresolved complex rearrangements involve 1p, 1q, 3cen, 9p, 11q, 12p, 12q, 16p, 17p, and 18cen. Moreover, a 4- to 6-fold gain of sequences on 18q leads to a low-level amplification of the BCL2 gene and to an overexpression of the BCL2 protein. These results provide the basis for the identification of not only candidate oncogenes responsible for MCL in gained regions, but also for the identification of putative tumor suppressor genes in commonly deleted regions like 1p22, which would eventually enable functional studies of these genes.
