Title of article :
Surface functionalization by poly-acrylic acid plasma-polymerized films for microarray DNA diagnostics
Author/Authors :
Ricciardi، نويسنده , , Serena and Castagna، نويسنده , , Riccardo and Severino، نويسنده , , Sara Maria and Ferrante، نويسنده , , Ivan and Frascella، نويسنده , , Francesca and Celasco، نويسنده , , Edvige and Mandracci، نويسنده , , Pietro and Vallini، نويسنده , , Ivan and Mantero، نويسنده , , Giovanni and Pirri، نويسنده , , Candido Fabrizio and Rivolo، نويسنده , , Paola، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Abstract :
The rapid detection of enteropathogens with high sensitivity and selectivity continues to be a significant challenge, especially in order to transfer laboratory analyses to the Point-Of‐Care (POC) by developing simple and reliable diagnostic kits. Bacterial infections are already detected, in analytical labs, by means of high-density microarray biochips based both on RNA/DNA fragments and antibodies as probes and antigens receptors, respectively, immobilized on the chip surface. Many efforts to obtain a high efficiency of the surface chemical properties for a stable probes grafting in order to avoid unspecificity or non-selectivity of the bio-recognition are still under way. The aim of this work is to show the advantages of applying a low pressure plasma polymerized acrylic acid (exposing COOH groups) thin coating to a low-density microarray biochip for the efficient grafting of suitable NH2 terminated probes able to match complementary DNA oligonucleotides of Listeria monocytogenes by means of a novel hybridization protocol and a commercial simple and low-cost colorimetric detection method compatible with POC applications.
emical properties of the obtained polyacrylic acid thin films are characterized by means of ATR FT-IR spectroscopy, XPS and contact angle (OCA) measurements. The surface density of the carboxylic functionalities is quantified by colorimetric titration with Toluidine Blue O (TBO).
timized functional thin film is shown to provide good advantages for DNA microarray diagnostics in terms of chemical stability, density of readily accessible COOH groups and at the same time low hydrophilicity, crucial for reducing the dilution of spotted probes on the surface and thus resulting in higher and satisfying intensity of the detect signals in the microarray test.
Keywords :
listeria , DNA low density microarray , plasma polymerization , Acrylic acid , ATR FT-IR , ESCA/XPS
Journal title :
Surface and Coatings Technology
Journal title :
Surface and Coatings Technology