IN-VITRO AND IN-SILICO SCREENING OF ANGIOTENSIN CONVERTING ENZYME ACTIVATOR; A SOLUTION FOR HYPOTENSION

The angiotensin converting enzyme (ACE) plays a vital role in regulating the blood pressure. The activity of ACE was screened between the eye and the heart of the zebrafish, by using hippuryl-L-histidyl-L-leusin as a substrate. Among these zebrafish eyes shows maximum activity. The screening of ACE activator is done from different plant methanol extract such as Salviniamolesta, Murrayakoenigii, Lantana camara & Eucalyptus oblique. Among these plants Lantanacamar a shows maximum ACE activity. The GC-MS analysis of Lantana camara detects 21 different compounds whereas cis-9-Octadecenal (19.8%), Squalene (19%), Hexadecanoic acid (17%) are in high concentration. All 21 compounds were subjected to the docking studies with an ACE enzyme using the software auto dock. Among these compounds Stigmast-5-en-ololeat shows maximum affinity towards the enzyme and this compound can be used as a potent ACE activator.