Author/Authors :
Szych، نويسنده , , Christine M. and Liesveld، نويسنده , , Jane L. and Iqbal، نويسنده , , M. Anwar and Li، نويسنده , , LiQiong and Siebert، نويسنده , , Susan and Asmus، نويسنده , , Courtney and OʹMalley، نويسنده , , Julie and Lee، نويسنده , , Adam and Wang، نويسنده , , Nancy، نويسنده ,
Abstract :
Amplification/duplication of the BCR-ABL gene has been found to be one of the key factors leading to drug resistance to imatinib mesylate (IM). In the present study, we used G-banding to identify the presence of de novo identical isodicentric chromosomes in IM-resistant patients. Fluorescence in situ hybridization (FISH) analysis on interphase nuclei confirmed the heterogeneity and amplification of the fused BCR-ABL gene. FISH analysis superimposed on G-banding confirmed the presence of isodicentric Philadelphia chromosomes. The impact of the isodicentric Philadelphia chromosomes on genomic instability, heterogeneity, and amplification of the Philadelphia chromosomes in IM-resistant patients is discussed.
