Title of article :
Molecular inversion probes reveal patterns of 9p21 deletion and copy number aberrations in childhood leukemia
Author/Authors :
Schiffman، نويسنده , , Joshua D. and Wang، نويسنده , , Yuker and McPherson، نويسنده , , Lisa A. and Welch، نويسنده , , Katrina and Zhang، نويسنده , , Nancy and Davis، نويسنده , , Ronald and Lacayo، نويسنده , , Norman J. and Dahl، نويسنده , , Gary V. and Faham، نويسنده , , Malek and Ford، نويسنده , , James M. and Ji، نويسنده , , Hanlee P.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
10
From page :
9
To page :
18
Abstract :
Childhood leukemia, which accounts for >30% of newly diagnosed childhood malignancies, is one of the leading causes of death for children with cancer. Genome-wide studies using microarray chips to identify copy number changes in human cancer are becoming more common. In this pilot study, 45 pediatric leukemia samples were analyzed for gene copy aberrations using novel molecular inversion probe (MIP) technology. Acute leukemia subtypes included precursor B-cell acute lymphoblastic leukemia (ALL) (n = 23), precursor T-cell ALL (n = 6), and acute myeloid leukemia (n = 14). The MIP analysis identified 69 regions of recurring copy number changes, of which 41 have not been identified with other DNA microarray platforms. Copy number gains and losses were validated in 98% of clinical karyotypes and 100% of fluorescence in situ hybridization studies available. We report unique patterns of copy number loss in samples with 9p21.3 (CDKN2A) deletion in the precursor B-cell ALL patients, compared with the precursor T-cell ALL patients. MIPs represent an attractive technology for identifying novel copy number aberrations, validating previously reported copy number changes, and translating molecular findings into clinically relevant targets for further investigation.
Journal title :
Cancer Genetics and Cytogenetics
Serial Year :
2009
Journal title :
Cancer Genetics and Cytogenetics
Record number :
1829830
Link To Document :
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