Author/Authors :
de Oliveira، نويسنده , , Fلbio Morato and Brandمo، نويسنده , , Renata Amorim and Leite-Cueva، نويسنده , , Sabrina Dias and de Paula Careta، نويسنده , , Francisco and Simُes، نويسنده , , Belinda Pinto and Rego، نويسنده , , Eduardo Magalhمes and Falcمo، نويسنده , , Roberto Passetto، نويسنده ,
Abstract :
We report a case of a 47-year-old man diagnosed with chronic lymphocytic leukemia (CLL) with two extra copies of chromosome 8. Classical cytogenetic analysis by the immunostimulatory combination of DSP30 and interleukin 2 showed tetrasomy of chromosome 8 in 60% of the metaphase cells (48,XY,+8,+8[12]/46,XY[8]). Spectral karyotype analysis confirmed the abnormality previously seen by G banding. Additionally, interphase fluorescence in situ hybridization using an LSI CEP 8 probe performed on peripheral blood cells without any stimulant agent showed tetrasomy of chromosome 8 in 54% of analyzed cells (108 of 200). To our knowledge, tetrasomy 8 as the sole chromosomal abnormality in CLL has not been previously described. The prognostic significance of tetrasomy 8 in CLL remains to be elucidated. However, the patient has been followed up in the outpatient hospital since 2004 without any therapeutic intervention and has so far remained stable.
