Title of article
Fluorescent oligonucleotide incorporating 5-(1-ethynylpyrenyl)-2′-deoxyuridine: sequence-specific fluorescence changes upon duplex formation
Author/Authors
Hwang، نويسنده , , Gil Tae and Seo، نويسنده , , Young Jun and Kim، نويسنده , , Su Jeong and Kim، نويسنده , , Byeang Hyean، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2004
Pages
4
From page
3543
To page
3546
Abstract
We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity.
Keywords
Nucleotides , fluorescence , nucleosides
Journal title
Tetrahedron Letters
Serial Year
2004
Journal title
Tetrahedron Letters
Record number
1841439
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