Variability in fibrosis in tissue samples obtained during diaphragmatic and apical LVAD implantation

AbstractBackground athologic study of left ventricular (LV) tissue can provide structural data on the heart at the time of left ventricular assist device (LVAD) implantation. We assessed the effect of cannula placement site (apical/diaphragmatic) and cardiomyopathy etiology (ischemic/nonischemic) on cardiac histopathologic changes. s heart failure patients, tissue was obtained from the standard apical cannula insertion site or the diaphragmatic surface site during implantation of the HeartMate II (n=53) or HeartWare (n=24) LVAD. Fibrosis and hypertrophy (cytoplasmic and nuclear diameter) were evaluated by computerized morphometry. s -two patients (27 men, 5 women; age, 57±15 years) underwent diaphragmatic implantation, and 45 (32 men, 13 women; age, 53±12 years) underwent apical implantation. The incidence of ischemic cardiomyopathy in the diaphragmatic and apical groups was 11/32 (34%) vs. 25/45 (56%) (P=.104). Overall, diaphragmatic tissue had less fibrosis than apical tissue (15.7±11.7% vs. 22.0±17.4%, P=.033), but both showed similar hypertrophic changes (cytoplasmic diameter, 39.0±10.3μm vs. 36.3±8.7μm, P=.141; nuclear diameter, 15.5±2.5μm vs. 14.8±3.0μm, P=.171). Likewise, in ischemic cardiomyopathy, apical samples showed more fibrosis than diaphragmatic samples (26.54±19.0% vs. 15.86±11.08%, P=.046) but similar hypertrophic changes (cytoplasmic diameter, 34.95±6.12μm vs. 37.48±12.07μm, P=.288; nuclear diameter, 14.66±2.69μm vs. 14.78±1.31μm, P=.451). sion dial histology results at the time of LVAD placement and their prognostic implications may be affected by inlet placement site and cardiomyopathy etiology. In this study, LV samples from apical implantation in ischemic cardiomyopathy patients were the most fibrotic. Thus, sampling site and cardiomyopathy etiology should be considered when interpreting LV samples obtained during LVAD implantation.