Adherent and non-adherent dendritic cells are equivalently qualified in GM-CSF, IL-4 and TNF-α culture system

We compared the purities, phenotype, capabilities of antigen uptake and T lymphocytes stimulation abilities of adherent and non-adherent cells of human monocyte-derived dendritic cells (mono-DCs) in GM-CSF, IL-4 and TNF-α culture system. The results show that both the purities and the capabilities of antigen uptake of the adherent DCs are significantly higher than those of non-adherent ones. As for the expression levels of surface markers and the abilities of stimulating lymphocytes proliferation, our results also show that those of adherent DCs are a bit higher than those of non-adherent ones, although the differences are not significant.