Detachment and Lysis of Adherent Target Cells by CD4+ T Cell Clones Involve Multiple Effector Mechanisms

Detachment of adherent targets from their substratum is a uniques activity of both CD81 and CD41 T cells. Recently, we have demonstrated that with mixed, alloreactive populations, DC41 T-cell-mediated detachment is kenitically distinct from lysis and requires protein synthesis. Heterogeneity of effector phenotypes precluded further elucidation of the mechanism of target cell detachment of adherent targets from their substratum (1) may result from either protein synthesis-dependent or independent pathways, which can be correlated with differences in clonal lytic phenotye, (2) is contact dependent and does not involve soluble factors. (3) can be pharmacologically uncoupled from IL4 production, as a measure of cytokine release, and (4) fails to correlate with perform expression by immunocytochemistry. Finally, isolated granule preparations are unable to mediate detachment from lysis.