Author/Authors :
Mao، نويسنده , , Jenny T. and Zhu، نويسنده , , Li X. and Sharma، نويسنده , , Sherven and Chen، نويسنده , , Ken L. Huang، نويسنده , , Min and Santiago، نويسنده , , S.Jonah and Gulsurd، نويسنده , , Jena and Tashkin، نويسنده , , Donald P. and Dubinett، نويسنده , , Steven M.، نويسنده ,
Abstract :
Cocaine use is associated with modulation of a broad range of biological functions including the capacity to influence cytokine production in murine and human immunoeffector cells. Little is known, however, regarding the effects of cocaine on endothelial cell cytokine production. Because the vascular endothelium actively participates in acute and chronic inflammatory responses and interleukin-8 (IL-8) is one of the key cytokines involved in the inflammatory process, modification of the production of IL-8 by vascular endothelial cells may interfere with the host response to infection or tissue injury. We investigated the effect of cocaine on endothelial cell IL-8 production. Conditioned supernatant from EA.hy 926 cells were evaluated by ELISA followingin vitrococaine exposure. Cocaine decreased IL-8 production in a dose-responsive manner, and this reduction correlated with down-regulation of IL-8 mRNA expression. Cocaine also increased the production of TGF-β by EA.hy 926 cells and anti-TGF-β abrogated the cocaine-mediated decrement of IL-8 production, indicating that cocaine down-regulates endothelial IL-8 production by increasing TGF-β. Our findings suggest that the immunomodulatory effects of cocaine may be mediated, in part, by modification of endothelial-derived cytokine production.
