Author/Authors :
Xu، نويسنده , , Junfa and Xiao، نويسنده , , Huan and Hu، نويسنده , , Guo-Yan and Zheng، نويسنده , , Shuhua and Liu، نويسنده , , Wei and Yuan، نويسنده , , Chun-Lei and Yang، نويسنده , , Heng and Lü، نويسنده , , Jing and Zheng، نويسنده , , Fang and Wang، نويسنده , , Cong-Yi and Gong، نويسنده , , Fei-Li، نويسنده ,
Abstract :
Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-γ and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-γ and IL-4 as well as enhanced IL-10 production.
Keywords :
B7-H4 , Concanavalin A , hydrodynamics- based gene delivery , Hepatic injury , IFN-? , IL-2 , IL-4 , IL-10
