Author/Authors :
Bosco، نويسنده , , Michaël and Gall، نويسنده , , Sophie Le and Rihouey، نويسنده , , Christophe and Couve-Bonnaire، نويسنده , , Samuel and Bardor، نويسنده , , Muriel and Lerouge، نويسنده , , Patrice and Pannecoucke، نويسنده , , Xavier، نويسنده ,
Abstract :
A new strategy to tag glycoproteins carrying terminal GlcNAc was developed using commercially available bovine β-1,4-galactosyltransferase (GalT) and UDP-6-azidogalactose. The azide function was then allowed to react via a biotinylated Staudinger–Bertozzi probe demonstrating the usefulness of such a procedure to tag any glycoprotein possessing a N-acetylglucosamine terminal residue from any type of cell lysate.
