Author/Authors :
Tan، نويسنده , , Xiaohong and Yang، نويسنده , , Renliang and Wirjo، نويسنده , , Andre and Liu، نويسنده , , Chuan-Fa، نويسنده ,
Abstract :
Subtiligase catalyzes the hydrolysis or the aminolysis of a peptide glycolate ester substrate via an acyl-enzyme thioester intermediate. We show that this intermediate can be intercepted by a hydrosulfide ion to generate a peptide thioacid as the hydrothiolysis product. Also shown is the use of the so-prepared peptide thioacids in mini thiol capture ligation.
