Author/Authors :
Amar B.T. Ghisaidoobe، نويسنده , , Amar B.T. and de Koning، نويسنده , , Martijn C. and Duynstee، نويسنده , , Howard I. and Ten Kortenaar، نويسنده , , Paul B.W. and Overkleeft، نويسنده , , Herman S. and Filippov، نويسنده , , Dmitri V. and van der Marel، نويسنده , , Gijs A.، نويسنده ,
Abstract :
A solution-phase approach for the synthesis of phosphorothioate oligonucleotides that circumvents the use of chromatographic purifications of protected phosphorothioate intermediates was developed. Implementation of a two-step sulfurization protocol in the phosphoramidite method allows efficient isolation of the intermediate phosphorothioates by extractions. The viability of this approach is demonstrated by the synthesis of a hexameric phosphorothioate oligonucleotide fragment.
