Author/Authors :
Alyamkina، نويسنده , , Ekaterina A. and Leplina، نويسنده , , Olga Y. and Sakhno، نويسنده , , Ludmila V. and Chernykh، نويسنده , , Elena R. and Ostanin، نويسنده , , Alexandr A. and Efremov، نويسنده , , Yaroslav R. and Shilov، نويسنده , , Alexandr G. and Proskurina، نويسنده , , Anastasia S. and Orishchenko، نويسنده , , Konstantin E. and Dolgova، نويسنده , , Evgenia V. and Rogachev، نويسنده , , Vladimir A. and Nikolin، نويسنده , , Valeriy P. and Popova، نويسنده , , Nelly A. and Zagrebelniy، نويسنده , , Stanislav N. and Bogachev، نويسنده , , Sergey S. and Shurdov، نويسنده , , Mikhail A.، نويسنده ,
Abstract :
A preparation of human genomic fragmented double-stranded DNA (dsDNA) was used as maturation stimulus in cultures of human dendritic cells (DCs) generated in compliance with the interferon protocol. Culturing of the DCs in medium with 5 μg/ml of the DNA preparation was associated with a decrease in the relative proportion of CD14 + cells and an increase in that of CD83 + cells. These changes are markers of DC maturation. The efficiency with which the DNA preparation was able to elicit DC maturation was commensurate with that of lypopolysaccharide from bacterial cell, the standard inducer of DC maturation. Generated ex vivo, matured in the presence of the human DNA preparation, pulsed with tumor antigens mouse DCs were used as a vaccine in biological tests for its antitumor activity. The experimental results demonstrate that reinfusion of mature pulsed with tumor antigens DCs cause a statistically significant suppression of tumor graft growth.
Keywords :
Nucleosome-free fragmented dsDNA , Mononuclear cells , CD14 , CD83 , Dendritic cell vaccine
