Author/Authors :
Liu، نويسنده , , Jingjing and He، نويسنده , , Chao and Zhou، نويسنده , , Hong and Xu، نويسنده , , Ya and Zhang، نويسنده , , Xiaolei and Yan، نويسنده , , Jinchuan and Xie، نويسنده , , Hongxiang and Cheng، نويسنده , , Si، نويسنده ,
Abstract :
Our previous study has demonstrated that Toll-like receptor 4 (TLR4) can contribute to anti-β2-glycoprotein I/β2-glycoprotein I (anti-β2GPI/β2GPI)-induced tissue factor (TF) expression in human acute monocytic leukemia cell line THP-1. However, the role of TLR4 in the activation of autoimmune response in antiphospholipid syndrome (APS) has rarely been reported. In this study, we focused on the role of TLR4 in β2GPI-induced maturation of bone marrow-derived dendritic cells (BMDCs). iDCs from C3H/HeN mice stimulated with β2GPI were more mature than that from C3H/HeJ mice, yields of CD11c+MHCII+DCs, CD11c+CD80+DCs and CD11c+CD86+DCs and production of some pro-inflammatory cytokines in iDCs from C3H/HeN were higher than those from C3H/HeJ (p < 0.05). Moreover, the ability of β2GPI-treated iDCs from C3H/HeJ to stimulate proliferation of allogeneic mixed lymphocytes was lower than that of iDCs from C3H/HeN. In conclusion, our results indicate that TLR4 may play a significant role in β2-glycoprotein I-induced BMDCs maturation.
