Author/Authors :
Abduallah، Ejaz نويسنده School of Business and Economics Abduallah, Ejaz , Hajjaj H.M. and Watanabe، نويسنده , , Kozo and Hayashizaki، نويسنده , , Koji and Iwayama، نويسنده , , Yuki and Takematsu، نويسنده , , Hiromu and Kozutsumi، نويسنده , , Yasunori and Tsubata، نويسنده , , Takeshi and Ishida، نويسنده , , Hideharu and Kiso، نويسنده , , Makoto، نويسنده ,
Abstract :
Biotin conjugate of the most potent ligand for mouse CD22 was designed and synthesized. The key synthetic step involved the dual capability of Hanessian reaction for debenzylation and simultaneous conversion of the anomeric p-methoxyphenyl group into the corresponding phenylthioglycoside in one step. Competition enzyme-linked immunosorbent assay (ELISA) for testing the binding affinity of synthetic sialosides was developed based on this biotinylated ligand.
