Author/Authors :
sereshti، h نويسنده Department of Chemistry, Faculty of Science, University of Tehran, Tehran, Iran , , bakhtiari، s نويسنده Department of Chemistry, Faculty of Science, University of Tehran, Tehran, Iran , , toghyani، r نويسنده Department of Chemistry, Faculty of Science, University of Tehran, Tehran, Iran , , samadi، s نويسنده Department of Chemistry, Faculty of Science, University of Tehran, Tehran, Iran , , Besharati-Seidani، t نويسنده Department of Chemistry, Faculty of Science, University of Guilan, 41335-1914, Rasht, Iran , , Mahmoodi، n.o نويسنده Department of Chemistry, Faculty of Science, University of Guilan, 41335-1914, Rasht, Iran ,
Abstract :
The ultrasound assisted emulsification microextraction (USAEME) method followed by
inductively coupled plasma-atomic emission spectrometry (ICP-AES) was used for preconcentration and
determination of bismuth (Bi), indium (In) and lead (Pb) in the plant Azolla filiculoides Lam. Calix [4] pyrrole
was used as a chelating agent and tetrachloroethylene was selected as extraction solvent. The main parameters
of the USAEME method were optimized by using response surface methodology (RSM). The optimal
conditions were as follows: 130 µL for volume of extraction solvent, 4 min for sonication time, 6.75 (w/v %)
for salt concentration, 890 mg/L for concentration of chelating agent and 7.5 for pH. The calibration graphs
were linear in the range of 1-1500 µg/L for Bi and In, and 1-1000 µg/L for Pb with the determination coefficients
(R2) of 0.9983, 0.9974 and 0.9997, respectively. The limits of detection (LODs) for Bi, In and Pb were 0.57,
0.76 and 0.84 µg/L, respectively. Under the optimal conditions, the method was successfully applied to the
determination of Bi, In and Pb in Azolla filiculoides Lam. tissues in Anzali wetland and satisfactory relative
recoveries (94–97%) were achieved.
