Author/Authors :
Uad، i نويسنده Departamento de Microbiolog?a, Facultad de Farmacia, Universidad de Granada, Campus Universitario de Cartuja, 18071 Granada, Spain , , Gonzalez-Lopez، j نويسنده Departamento de Microbiolog?a, Facultad de Farmacia, Universidad de Granada, Campus Universitario de Cartuja, 18071 Granada, Spain , , Silva-Castro، g.a نويسنده Departamento de Microbiolog?a, Facultad de Farmacia, Universidad de Granada, Campus Universitario de Cartuja, 18071 Granada, Spain , , V?lchez، j.i نويسنده Departamento de Microbiolog?a, Facultad de Farmacia, Universidad de Granada, Campus Universitario de Cartuja, 18071 Granada, Spain , , Gonzalez-Martinez، a نويسنده Departamento de Ingenier?a Civil, ETS Ingenieros de Caminos Canales y Puertos, Universidad de Granada, Campus de Fuentenueva, 18071 Granada, Spain , , Martin-Ramos، d نويسنده Departamento de Mineralog?a y Petrolog?a, Facultad de Ciencias, Universidad de Granada, Campus Universitario de Fuentenueva, 18071 Granada, Spain , , Rivadeneyra، a نويسنده Departamento de Electr?nica y Tecnolog?a de Computadores, Escuela Técnica Superior de Ingenier?a Informatica y de Telecomunicaci?n, Universidad de Granada, Campus Universitario de Almanjayar, 18071 Granada, Spain , , Rivadeneyra، m.a نويسنده Departamento de Microbiolog?a, Facultad de Farmacia, Universidad de Granada, Campus Universitario de Cartuja, 18071 Granada, Spain ,
Abstract :
The precipitation of calcium carbonate by bacteria isolated from submerged fixed-film bioreactor
used for the treatment of urban wastewater in both natural and artificial media was studied. However, carbonate
precipitation was detected only when the bacteria grow in media added with calcium. Precipitation took place
rapidly, and crystal formation began 3 d after inoculation and the percentage of carbonate-forming bacteria was
slightly higher than 90%. Seventeen dominant colonies with carbonate precipitation capacity were the
dominant heterotrophic platable bacteria growing aerobically in media added with calcium acetate and calcium
chloride. According to their taxonomic affiliations (based on partial sequencing of the 16S-rRNA), the seventeen
strains belonged to the following genera of Gram-positive and Gram-negative bacteria: Artrobacter, Bacillus,
Enterococcus, Pseudomonas, Agromyces and Rhodococcus. All of these strains formed calcium carbonate
crystals, precipitated always as calcite crystals which showed large amounts of bacterial fingerprints or
bacteria calcification. In addition all the seventeen bacterial strains showed carbonic anhydrase activity. The
results of this research suggest that in submerged fixed-film bioreactors used for the treatment of urban
wastewater, the precipitation of carbonates through bacterial action could take place in situ in the presence of
certain concentrations of calcium. Moreover, this biological activity could be useful in the CO2 and calcium
capture in urban wastewater treatments.
