Author/Authors :
Avitabile، نويسنده , , Concetta and Moggio، نويسنده , , Loredana and D’Andrea، نويسنده , , Luca D. and Pedone، نويسنده , , Carlo and Romanelli، نويسنده , , Alessandra، نويسنده ,
Abstract :
An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%.
Keywords :
peptide nucleic acid , Manual synthesis , FMOC , pyridine
