Author/Authors :
Nawalany، نويسنده , , Kinga and Rusin، نويسنده , , Aleksandra and K?pczy?ski، نويسنده , , Mariusz and Mikhailov، نويسنده , , Alexei and Kramer-Marek، نويسنده , , Gabriela and ?nietura، نويسنده , , Miros?aw and Po?towicz، نويسنده , , Jan and Krawczyk، نويسنده , , Zdzis?aw and Nowakowska، نويسنده , , Maria، نويسنده ,
Abstract :
Two photosensitizing systems: (1) tetrakis(4-hydroxyphenyl)porphyrin (p-THPP) encapsulated in sterically stabilized liposomes (SSL) and (2) p-THPP functionalized by covalent attachment of poly(ethylene glycol) (p-THPP–PEG2000) were studied in vitro. The dark and photo cytotoxicity of these systems were evaluated on two cell lines: HCT 116, a human colorectal carcinoma cell line, and DU 145, a prostate cancer cell line and compared with these determined for free p-THPP. It was demonstrated that both encapsulation in liposomes as well as attachment of PEG chain result in pronounced reduction of the dark cytotoxicity of the parent porphyrin. The liposomal formulation showed higher than p-THPP–PEG2000 photocytotoxicity towards both cell lines used in the studies.
Keywords :
photodynamic therapy , Porphyrins , Sterically stabilized liposomes , Cancer cells
