Title of article :
Simultaneous binding of anti-tuberculosis and anti-thrombosis drugs to a human transporter protein: A FRET study
Author/Authors :
Rajdev، نويسنده , , Priya and Mondol، نويسنده , , Tanumoy and Makhal، نويسنده , , Abhinandan and Pal، نويسنده , , Samir Kumar، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
6
From page :
153
To page :
158
Abstract :
Although rifampicin (Rf) is one of the most effective antibiotics against infection caused by Mycobacterium tuberculosis, interaction of the drug with universal carrier protein in human blood plasma is not fully understood. Reduction of medicinal efficacy of other drugs, including anti-thrombosis drug warfarin (Wf), to the patients on Rf therapy also needs molecular understanding. In the present work we have studied interaction of Rf with one of the model carrier protein (human serum albumin). By using circular dichroism (CD) spectroscopy we have characterized the change in the secondary structure of the protein. The consequence of the simultaneous binding of the two drugs, Rf and Wf, on the structure of the protein has also been explored. Picosecond resolved Fِrster resonance energy transfer (FRET) from Wf to Rf explores possible binding sites of the anti-tuberculosis drug on the protein. In this report, we have discussed the potential problem of using the single tryptophan of the protein (Trp 214) as energy donor in FRET experiment for the characterization of the binding site of the drug Rf on the protein.
Keywords :
Rifampicin , Binding interaction with transporter protein , Warfarin , FRET , TCSPC , CD
Journal title :
Journal of Photochemistry and Photobiology B:Biology
Serial Year :
2011
Journal title :
Journal of Photochemistry and Photobiology B:Biology
Record number :
1877361
Link To Document :
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