Title of article :
Contrasting binding of fisetin and daidzein in γ-cyclodextrin nanocavity
Author/Authors :
Pahari، نويسنده , , Biswapathik and Sengupta، نويسنده , , Bidisha and Chakraborty، نويسنده , , Sandipan and Thomas، نويسنده , , Briannica and McGowan، نويسنده , , Dyffreyon and Sengupta، نويسنده , , Pradeep K.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Abstract :
Steady state and time resolved fluorescence along with anisotropy and induced circular dichroism (ICD) spectroscopy provide useful tools to observe and understand the behavior of the therapeutically important plant flavonoids fisetin and daidzein in γ-cyclodextrin (γ-CDx) nanocavity. Benesi–Hildebrand plots indicated 1:1 stoichiometry for both the supramolecular complexes. However, the mode of the binding of fisetin significantly differs from daidzein in γ-CDx, as is observed from ICD spectra which is further confirmed by docking studies. The interaction with γ-CDx proceeds mainly by the phenyl ring and partly by the chromone ring of fisetin whereas only the phenyl ring takes part for daidzein. A linear increase in the aqueous solubility of the flavonoids is assessed from the increase in the binding of the flavonoids with the γ-CDx cavity, which are determined by the gradual increase in the ICD signal, fluorescence emission as well as increase in fluorescence anisotropy with increasing (γ-CDx). This confirms γ-CDx as a nanovehicle for the flavonoids fisetin and daidzein in improving their bioavailability.
Keywords :
Excited-State Intramolecular Proton Transfer , Steady state and Time resolved fluorescence spectroscopy , Fluorescence anisotropy , molecular docking , Induced circular dichroism
Journal title :
Journal of Photochemistry and Photobiology B:Biology
Journal title :
Journal of Photochemistry and Photobiology B:Biology