Author/Authors :
Garcيa-Beltrلn، نويسنده , , Olimpo and Mena، نويسنده , , Natalia and Pérez، نويسنده , , Edwin G. and Cassels، نويسنده , , Bruce K. and Nuٌez، نويسنده , , Marco T. and Werlinger، نويسنده , , Francisca and Zavala، نويسنده , , Daniel and Aliaga، نويسنده , , Margarita E. and Pavez، نويسنده , , Paulina، نويسنده ,
Abstract :
Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols toward both probes (ChC1 and ChC2) follows the order Cys > GSH > Hcy > Cys-Gly, ChC1 being less reactive than ChC2. Possible interference with other amino acids was assessed. ChC1 and ChC2 display a highly selective fluorescence enhancement with thiols, allowing these probes to be used for fluorimetric thiol determination in SH-SY5Y cells.
Keywords :
Michael addition , Fluorescence probes , Coumarin-based derivatives , Biothiols
